E. coli genotypesFrom OpenWetWareContents1 Nomenclature & Abbreviations2 Methylation Issues in E. coli3 Commonly used strains3.1 AG13.2 AB11573.3 B21553.4 BL213.5 BL21(AI)3.6 BL21(DE3)3.7 BL21 (DE3) pLysS3.8 BNN933.9 BNN973.10 BW26434, CGSC Strain # 76583.11 C6003.12 C600 hflA150 (Y1073, BNN102)3.13 CSH503.14 D12103.15 DB3.13.16 DH13.17 DH5α3.18 DH5α Turbo (NEB)3.19 DH10B (Invitrogen)3.20 DH12S (Invitrogen)3.21 DM1 (Invitrogen)3.22 E. cloni(r) 5alpha (Lucigen)3.23 E. cloni(r) 10G (Lucigen)3.24 E. cloni(r) 10GF' (Lucigen)3.25 E. coli K12 ER2738 (NEB)3.26 ER2566 (NEB)3.27 ER2267 (NEB)3.28 HB1013.29 HMS174(DE3)3.30 High-Control(tm) BL21(DE3) (Lucigen)3.31 High-Control(tm) 10G (Lucigen)3.32 IJ11263.33 IJ11273.34 JM833.35 JM1013.36 JM1033.37 JM1053.38 JM1063.39 JM1073.40 JM1083.41 JM1093.42 JM109(DE3)3.43 JM1103.44 JM2.3003.45 LE3923.46 M15 (Qiagen)3.47 Mach13.48 MC10613.49 MC41003.50 MFDpir3.51 MG16553.52 OmniMAX23.53 OverExpress(tm)C41(DE3) (Lucigen)3.54 OverExpress(tm)C41(DE3)pLysS (Lucigen)3.55 OverExpress(tm)C43(DE3) (Lucigen)3.56 OverExpress(tm)C43(DE3)pLysS (Lucigen)3.57 Rosetta(DE3)pLysS3.58 Rosetta-gami(DE3)pLysS3.59 RR13.60 RV3083.61 SOLR (Stratagene)3.62 SS320 (Lucigen)3.63 STBL2 (Invitrogen)3.64 STBL3 (Invitrogen)3.65 STBL43.66 SURE (Stratagene)3.67 SURE2 (Stratagene)3.68 TG1 (Lucigen)3.69 TOP10 (Invitrogen)3.70 Top10F' (Invitrogen)3.71 W31103.72 W3110 (λ857S7)3.73 WM3064http://openwetware.org/wiki/E._coli_genotypes1/142015/5/26E. coli genotypes OpenWetWare3.74 XL1-Blue (Stratagene)3.75 XL1-Blue MRF' (Stratagene)3.76 XL2-Blue (Stratagene)3.77 XL2-Blue MRF' (Stratagene)3.78 XL1-Red (Stratagene)3.79 XL10-Gold (Stratagene)3.80 XL10-Gold KanR (Stratagene)4 Other genotype information sources5 ReferencesNomenclature & AbbreviationsA listed gene name means that gene carries a loss of function mutation, a Δ preceding a gene name means the gene is deleted. If agene is not listed, it is not known to be mutated. Prophages present in wt K-12 strains (F, λ, e14, rac) are listed only if absent.E. coli B strains are naturally lon- and dcm-.F- = Does not carry the F plasmidF+ = Carries the F plasmid. The cell is able to mate with F- through conjugation.F'[ ] = Carries an F plasmid that has host chromosomal genes on it from a previous recombination event. This cell can also matewith F- through conjugation. Chromosomal genes carried in the F plasmid are listed in brackets.rB/K+/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the restriction system.mB/K+/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the modification(methylation) system.hsdS = Both restriction and methylation of certain sequences is deleted from the strain. If you transform DNA from such a straininto a wild type strain, it will be degraded.hsdR = For efficient transformation of cloned unmethylated DNA from PCR amplificationsINV( ) = chromosomal inversion between locations indicatedahpC = mutation to alkyl hydroperoxide reductase conferring disulfide reductase activityara-14 = cannot metabolize arabinosearaD = mutation in L-ribulose-phosphate 4-epimerase blocks arabinose metabolismcycA = mutation in alanine transporter; cannot use alanine as a carbon sourcedapD = mutation in succinyl diaminopimelate aminotransferase leads to succinate or (lysine + methionine) requirementΔ( ) = chromosomal deletion of genes between the listed genes (may include unlisted genes!)dam = adenine methylation at GATC sequences exist; high recombination efficiency; DNA repair turned ondcm = cytosine methylation at second C of CCWGG sites exist. dam & dcm are the default properties and always elided, while dam-or dcm- should be declare explicitlyDE3 (http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/Transformation/expression-competent-cells.html) = Lysogen that encodes T7 RNA polymerase. Used to induce expression in T7-driven expression systemsdeoR = regulatory gene that allows constitutive expression of deoxyribose synthesis genes; permits uptake of large plasmids. SeeHanahan D, US Patent 4,851,348 (http://patft.uspto.gov/netacgi/nph-Parser?u=/netahtml/srchnum.htm&Sect1=PTO1&Sect2=HITOFF&p=1&r=1&l=50&f=G&d=PALL&s1=4851348.WKU.&OS=PN/4851348&RS=PN/4851348) . ***Thishas been called into question, as the DH10B genome sequence revealed that it is deoR+. See Durfee08, PMID 18245285.dnaJ = one of the chaparonins inactivated; stabilizes some mutant proteinsdut1 = dUTPase activity abolished, leading to increased dUTP concentrations, allowing uracil instead of thymine incorporation inDNA. Stable U incorporation requires ung gene mutation as well.endA1 = For cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specificdigestion by Endonuclease I(e14) = excisable prophage like element containing mcrA gene; present in K-12 but missing in many other strainsgalE = mutations are associated with high competence, increased resistance to phage P1 infection, and 2-deoxygalactoseresistance. galE mutations block the production of UDP-galactose, resulting in truncation of LPS glycans to the minimal, \"innercore\". The exceptional competence of DH10B/TOP10 is thought to be a result of a reduced interference from LPS in the bindingand/or uptake of transforming DNA. galE15 is a point mutation resulting in a Ser123 -> Phe conversion near the enzyme's activesite. See van Die, et al. PMID 6373734, Hanahan, et al. PMID 1943786, and EcoSal ISBN 1555811647. --Dcekiert 16:56, 23 January2008 (CST)galk = mutants cannot metabolize galactose and are resistant to 2-deoxygalactose. galK16 is an IS2 insertion ~170bp downstream ofthe galK start codon. See EcoSal ISBN 1555811647. --Dcekiert 16:56, 23 January 2008 (CST)galU = mutants cannot metabolize galactosegor = mutation in glutathione reductase; enhances disulphide bond formationglnV = suppression of amber (UAG) stop codons by insertion of glutamine; required for some phage growthgyrA96 = mutation in DNA gyrase; conveys nalidixic acid resistancegyrA462 = mutation in DNA gyrase; conveys resistance to ccdB colicin gene producthflA150 = protease mutation stabilizing phage cII protein; high frequency of lysogenization by λΔ(lac)X74 = Deletion of the entire lac operon as well as some flanking DNA (complete deletion is Δcod-mhpF; see Mol.Micro.,6:1335, and J.Bact., 179:2573)lacIq or lacIQ = overproduction of the lac repressor protein; -35 site in promoter upstream of lacI is mutated from GCGCAA toGTGCAAlacIQ1 = overproduction of the lac repressor protein; contains a 15 bp deletion to create optimal -35 site in promoter upstreamof lacIlacY = deficient in lactose transport; deletion of lactose permease (M protein)lacZΔM15 = partial deletion of the lacZ gene that allows α complementation of the β-galactosidase gene; required forblue/white selection on XGal plates. Deletes the amino portion of lacZ (aa 11-41).LAM- or λ- = lambda lysogen deletion; approximate map location: 17.40; information from CGSC(http://cgsc.biology.yale.edu/Mutation.php?ID=4499) *---Karmella 13:02, 21 October 2012 (EDT):LamR = mutation in malT1 conferring lambda resistance; synonym malT1(LamR) [1] (http://cgsc.biology.yale.edu/Mutation.php?ID=4749) *---Karmella 13:35, 21 October 2012 (EDT):leuB = requires leucineΔlon = deletion of the lon proteasemalA = cannot metabolize maltosemcrA = Mutation eliminating restriction of DNA methylated at the sequence CmCGG (possibly mCG). Carried on the e14 prophage(q.v.)mcrB = Mutation eliminating restriction of DNA methylated at the sequence RmChttp://openwetware.org/wiki/E._coli_genotypes2/142015/5/26E. coli genotypes OpenWetWare
metB = requires methioninemetC = requires methionine
mrr = Mutation eliminating restriction of DNA methylated at the sequence CmAG or GmACmtlA = cannot metabilize mannitol
(Mu) = Mu prophage present. Muδ means the phage is defective.
mutS - mutation inhibits DNA repair of mismatches in unmethylated newly synthesized strandsnupG = same as deoR
ompT = mutation in outer membrane protein protease VII, reducing proteolysis of expressed proteins(P1) = Cell carries a P1 prophage. Cells express the P1 restriction system.(P2) = Cell carries a P2 prophage. Allows selection against Red+ Gam+ λ
(φ80) = Cell carries the lambdoid prophage φ80. A defective version of this phage carrying lacZM15 deletion (as well as wild-type lacI, lacYA, and flanking sequences) is present in some strains. The φ80 attachment site is just adjacent to tonB.
pLysS = contains pLysS plasmid carrying chloramphenicol resistance and phage T7 lysozyme, effective at attenuating activity of T7RNA polymerase, for better inhibition of expression under non-induced conditions. The sequence can be found here(http://www.emdbiosciences.com/docs/NDIS/69659-000.HTML) .proA/B = requires proline
recA1 = For reduced occurrence of unwanted recombination in cloned DNA; cells UV sensitive, deficient in DNA repairrecA13 = as for recA1, but inserts less stable.
recBCD = Exonuclease V; mutation in RecB or RecC reduces general recombination by a factor of 100; impaired DNA repair; UVsensitive, easier propagation of inverted repeatsrecJ Exonuclease involved in alternate recombination
relA = relaxed phenotype; permits RNA synthesis in absence of protein synthesisrha = blocked rhamose metabolism
rnc = encodes RnaseIII (rnc-14 is a common null mutant)
rne = encodes RnaseE (rne-3071 is a common temperature sensitive mutant)
rpsL = mutation in ribosomal protein S12 conveying streptomycin resistance; also called strA, rpsL135(strR), strA135 [2](http://cgsc.biology.yale.edu/Mutation.php?ID=5280) *---Karmella 13:27, 21 October 2012 (EDT):
sbcBC = ExoI activity abolished; usually present in recBC strains; recombination proficient, stable inverted repeatssr1 = cannot metabolize sorbitolsupE = glnVsupF = tyrT
thi = requires thiaminethyA = requires thymidine
Tn10 = transposon normally carrying Tetracycline resistanceTn5 = transposon normally carrying Kanamycin resistance
tonA = Mutation in outer membrane protein conveying resistance to phage T1 and phage T5traD = Mutation eliminating transfer factor; prevents transfer of F plasmid
trxB = mutation in thioredoxin reductase; enhances disulphide bond formation in the cytoplasmtsx = outer membrane protein mutation conveying resistance to phage T6 and colicin K
tyrT = suppression of amber (UAG) stop codons by insertion of tyrosine; needed for some phage infection such as λgt11.ung1 = allows uracil to exist in plasmid DNAxyl-5 = blocked xylose metabolismSmR = Streptomycin resistance
Methylation Issues in E. coli
Type I methylation systems:
E. coli K12 restricts DNA which is not protected by adenine methylation at sites AA*C[N6]GTGC or GCA*C[N6]GTT, encoded bythe hsdRMS genes(EcoKI). Deletions in these genes removes either the restriction or methylation or both of these functions.E. coli B derivative strains contain an hsdRMS system (EcoBI) restricting and protecting the sequence TGA*[N8]TGCT or AGCA*[N8]TCA.The mcrA gene (carried on the e14 prophage) restricts DNA which is methylated in CmCWGG or mCG sequences (methylation by the dcmgene product).
The mcrBC genes restrict RmC sequences.
The mrr gene product restricts adenine methylated sequences at CAG or GAC sites.
E. coli methylates the adenine in GATC (and the corresponding A on the opposite strand) with the dam gene product.
M.EcoKII methylates the first A at the palindromic site ATGCAT (as well as the corresponding A on the opposite strand), see(Kossykh VG (2004) J. Bact 186: 2061-2067 PMID 15028690) Note that this article has been retracted; the retraction appears tocenter on textual plagarism, not experimental results. The homology to AvaIII is real. I think I believe it. tk 20:28, 9 December2005 (EST). Rich Roberts reports: \"We have tried ourselves to detect activity with this gene product and cannot detect any
methyltransferase activity. In our case we used antibodies able to detect N6-methyladenine or N4 methylcytosine in DNA. The oneswe have are very sensitive and should have been able to detect 5-methyl groups in the whole E. coli chromosome. Nothing wasdetected in an over-expressing strain.\"
For additional information see E. coli restriction-modification system and the NEB technical information on methylation(http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/making_unmethylated_dna.asp) .
Commonly used strains
AG1
endA1 recA1 gyrA96 thi-1 relA1 glnV44 hsdR17(rK- mK+)
AB1157
thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnV44(AS), galK2(Oc), LAM-, Rac-0, hisG4(Oc), rfbC1, mgl-51,rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thi-1
Bachmann BJ: Derivation and genotypes of some mutant derivatives of Escherichia coli K-12.
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Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology (Edited by: F C Neidhardt J L Ingraham KB Low B MagasanikM Schaechter H E Umbarger). Washington, D.C., American Society for Microbiology 1987, 2:1190-1219.See CGSC#1157 (http://cgsc2.biology.yale.edu/Strain.php?ID=4509)
B2155
thrB1004 pro thi strA hsdsS lacZD M15 (F`lacZD M15 lacIq traD36 proA+ proB+) D dapA::erm (Ermr) pir::RP4 [::kan (Kmr) from SM10]An E. coli strain carrying the pir sequence required for maintenance of plasmids containing R6K ori. Also, this strain is auxotrophicfor DAP (diaminopimelic acid - a lysine precursor). The auxotrophy helps in removal of this strain from a bi-parental mating setupafter conjugation.
Ref: Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselaefollowing conjugal plasmid transfer from Escherichia coliDehio, C. & Meyer, M. (1997) J. Bacteriol. 179, 538–540
BL21
E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)
The \"malB region\" was transduced in from the K-12 strain W3110 to make the strain Mal+λS. See Studier et al. (2009) J. Mol.Biol. 394(4), 653 for a discussion of the extent of the transfer.
Stratagene E. coli Genotype Strains (http://www.stratagene.com/pdf/mobio/E%20Coli_Genotype%20Strains.pdf)
BL21(AI)
F– ompT gal dcm lon hsdSB(rB- mB-) araB::T7RNAP-tetA
an E. coli B strain carrying the T7 RNA polymerase gene in the araB locus of the araBAD operonq.
Transformed plasmids containing T7 promoter driven expression are repressed until L-arabinose induction of T7 RNA polymerase.
Maximal expression is lower than that of BL21(DE3) (customer support 10/2012)Derived from BL21.
See the product page (https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=595)for more information.
Brian Caliendo (Voigt lab) reported trouble getting the Datsenko and Wanner (2000) plasmid pCP20 to transform into this strain,when other strains transformed fine. Cause is unknown.
BL21(DE3)
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
Derived from B834 (Wood, 1966 (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=File&DB=pubmed) ) by transducing to Met+.See the original Studier paper (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?
cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3537305&query_hl=14) or the summary in Methods in Enzymology(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?
cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796&query_hl=13&itool=pubmed_docsum) for more details.Whole genome sequence available [3] (http://www.ncbi.nlm.nih.gov/genomes/lproks.cgi)
BL21 (DE3) pLysS
F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR)
pLysS plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmidChloramphenicol resistant
The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression fromtransformed T7 promoter containing plasmids when not induced.see Moffatt87 for details of pLysS and pLysE plasmids
BNN93
F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 mcrB e14-(mcrA-) hsdR(rK-mK+) λ-Some C600 strains are really BNN93
BNN97
BNN93 (λgt11)
A λgt11 lysogen producing phage at 42C
BW26434, CGSC Strain # 7658
Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514
This information is from a printout sent by the E. coli Genetic Stock Center (http://cgsc.biology.yale.edu) with the strain.B.L. Wanner strain
rph-1 is a 1bp deletion that results in a frameshift over last 15 codons and has a polar effect on pyrE leading to suboptimalpyrimidine levels on minimal medium. (Jensen 1993 J Bact. 175:3401)
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Δ(araD-araB)567 was formerly called ΔaraBADAH33 by Datsenko and WannerAm = amber(UAG) mutation
Reference: Datsenko and Wanner, 2000, PNAS, 97:6640NOTE:
This promoter driving the expression of lacI was sequenced in this strain using a primer in mhpR (upstream of lacI) and a primerin the opposite orientation in lacI. The lac promoter was found to be identical to wildtype. Thus, the -35 sequence was GCGCAAnot GTGCAA as expected with lacIq. Therefore this strain (or at least the version obtained from the E. coli Genetic Stock Center(http://cgsc.biology.yale.edu) ) does NOT appear to be lacIq. According to Barry Wanner, this is an unexpected result. -Reshma13:19, 5 May 2005 (EDT)
\"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the errorwith respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.\" (from Barry WannerNovember 18, 2005)
The genotype has been corrected at the CGSC (http://cgsc2.biology.yale.edu/Strain.php?ID=65553)
C600
F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 λ-There are strains circulating with both e14+(mcrA+) and e14-(mcrA-)General purpose host
See CGSC#3004 (http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/11195)
References: Appleyard, R.K. (1954) Genetics 39, 440; Hanahan, D. (1983) J. Mol. Biol. 166, 577.
C600 hflA150 (Y1073, BNN102)
F- thi-1 thr-1 leuB6 lacY1 tonA21 glnV44 λ- hflA150(chr::Tn10)
host for repressing plaques of λgt10 when establishing cDNA libraries
Reference Young R.A. and Davis, R. (1983) Proc. Natl. Acad. Sci. USA 80, 1194.Tetracycline resistance from the Tn10 insertion
CSH50
F- λ- ara Δ(lac-pro) rpsL thi fimE::IS1
See CGSC#8085 (http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/28630)
References: Miller, J.H. 1972. Expts.in Molec.Genetics, CSH 0:14-0; Blomfeld et al., J.Bact. 173: 5298-5307, 1991.
D1210
HB101 lacIq lacY+
DB3.1
F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1
useful for propagating plasmids containing the ccdB operon.gyrA462 enables ccdB containing plasmid propagationstreptomycin resistant
appears to NOT contain lacI (based on a colony PCR) --Austin Che 16:16, 18 June 2007 (EDT)1. Bernard P and Couturier M. . pmid:1324324.
2. Miki T, Park JA, Nagao K, Murayama N, and Horiuchi T. . pmid:1316444.
DH1
endA1 recA1 gyrA96 thi-1 glnV44 relA1 hsdR17(rK- mK+) λ-parent of DH5α
An Hoffman-Berling 1100 strain derivative (Meselson68)more efficient at transforming large (40-60Kb) plasmidsnalidixic acid resistant
Reference: Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
DH5α
F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ–
An Hoffman-Berling 1100 strain derivative (Meselson68)Promega also lists phoAnalidixic acid resistantReferences:
FOCUS (1986) 8:2, 9.
Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
DH5α Turbo (NEB)
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F´ proA+B+ lacIq ∆ lacZ M15/ fhuA2 ∆(lac-proAB) glnV gal R(zgb-210::Tn10)TetS endA1 thi-1 ∆(hsdS-mcrB)5
Also known as NEB TurboT1 phage resistant
Rapid growth: visible colonies on agar, ~6.5 hours; shaking liquid culture OD 600 = 2.0, ~4 hoursExpresses the Lac repressorReferences:
New England Biolabs, product catalogue number C2984H
DH10B (Invitrogen)
F- endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-suitable for cloning methylated cytosine or adenine containing DNA
an MC1061 derivative (Casadaban80). Prepare cells for chemical transformation with CCMB80 bufferblue/white selection
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B (and bytheir lineage also TOP10 and any other MC1061 derivatives) is actually galE galK galU+. Dcekiert 16:37, 23 January 2008 (CST)Genome sequence indicates that DH10B is actually deoR+. Presumably TOP10 and MC1061 are also deoR+.Streptomycin resistantleucine auxotrophReferences:
Casdaban, M. and Cohen, S. (1980) J Mol Biol 138:179 PMID 6997493.
Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
E. coli Genetic Stock Center, MC1061 Record (http://cgsc2.biology.yale.edu/Strain.php?ID=11225)
DH10B Genome Sequencing Project, Baylor College of Medicine (http://www.hgsc.bcm.tmc.edu/projects/microbial/microbial-detail.xsp?project_id=105)
Complete sequence is available, see Durfee08, PMID 18245285.
DH12S (Invitrogen)
mcrA Δ(mrr-hsdRMS-mcrBC) φ80d lacZΔM15 ΔlacX74 recA1 deoR Δ(ara, leu)7697 araD139 galU galK rpsL F' [proAB+ lacIqZΔM15]
host for phagemid and M13 vectors
useful for generating genomic libraries containing methylated cytosine or adenine residuesstreptomycin resistant
References: Lin, J.J., Smith, M., Jessee, J., and Bloom, F. (1991) FOCUS 13, 96.; Lin, J.J., Smith, M., Jessee, J., and Bloom, F.(1992) BioTechniques 12, 718.
DM1 (Invitrogen)
F- dam-13::Tn9(CmR) dcm- mcrB hsdR-M+ gal1 gal2 ara- lac- thr- leu- tonR tsxR Su0
Host for pBR322 and other non-pUC19 plasmids; useful for generating plasmids that can be cleaved with dam and dcm sensitiveenzymes
Chloramphenicol resistantPromega lists as F' not F-Reference: Lorow-Murray D and Bloom F (1991) Focus 13:20
E. cloni(r) 5alpha (Lucigen)
fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
Common cloning strain.
E. cloni(r) 10G (Lucigen)
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
Common cloning strain.Resistant to phage T1.
E. cloni(r) 10GF' (Lucigen)
[F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galUgalK rpsL nupGλ tonA
Strain for cloning and single-strand DNA production.
E. coli K12 ER2738 (NEB)
F´proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5
Phage propagation strain
Also available from Lucigen Corporation.
ER2566 (NEB)
F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm]
Host strain for the expression of a target gene cloned in the pTYB vectors.
Carry a chromosomal copy of the T7 RNA polymerase gene inserted into lacZ gene and thus under the control of the lac promoter. Inthe absence of IPTG induction expression of T7 RNA polymerase is suppressed by the binding of lac I repressor to the lac
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promoter.
Deficient in both lon and ompT proteases.
ER2267 (NEB)
F´ proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14-(McrA-) rfbD1? recA1 relA1? endA1 spoT1? thi-1Δ(mcrC-mrr)114::IS10
Commonly used for titering M13 phage because of the strain's F' plasmid, which carries KanR, and its slow growth, which promoteseasy visualization of plaques.
HB101
F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-Please note that different sources have different genotypes so treat this information with caution.
From a GIBCO BRL list of competent cells.
Hybrid of E. coli K12 and E. coli B (but 98% K strain AB266 according to Smith et al.)Host for pBR322 and many plasmids
Sigma lists the deletion Δ(gpt,proA). Check this.Promega does not list F-, mcrB, or mrrStreptomycin resistantReferences:
Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56 - pdf version
(http://www.invitrogen.com/Content/Focus/Focus%20Volume%2011%20Issue%203.PDF) from InvitrogenLacks S and Greenberg JR (1977) J Mol Biol 114:153.
HMS174(DE3)
F- recA1 hsdR(rK12- mK12+) (DE3) (Rif R)
HMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whoseproducts may cause the loss of the DE3 prophage.
DE3 indicates that the host is a lysogen of lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene undercontrol of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors byinduction with IPTG.
Full genome sequence available LM993812 (http://www.ebi.ac.uk/ena/data/view/LM993812/) *J Mairhofer 07:36, 28 October 2014(EDT):.
High-Control(tm) BL21(DE3) (Lucigen)
F– ompT gal dcm hsdSB(rB- mB-) (DE3)/Mini-F lacIq1(Gentr)
The HI-Control BL21(DE3) cells contain a single-copy BAC plasmid harboring a specially engineered version of the lacIq1 repressorallele. The lacIq1 allele expresses ~170-fold more lac repressor protein than the wild-type lacI gene.
The increased pool of lac repressor in HI-Control BL21(DE3) cells maintains tight control over the expression of T7 RNApolymerase from the lacUV5 promoter, reducing leaky expression of genes cloned under a T7 promoter.an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
High-Control(tm) 10G (Lucigen)
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA/Mini-FlacIq1(Gentr)
The HI-Control 10G cells contain a single-copy BAC plasmid harboring a specially engineered version of the lacIq1 repressorallele. The lacIq1 allele expresses ~170-fold more lac repressor protein than the wild-type lacI gene.For stable cloning of T7 protein expression plasmids.Resistant to phage T1.
IJ1126
E. coli K-12 recB21 recC22 sbcA5 endA gal thi Su+ Δ(mcrC-mrr)102::Tn10See Endy:IJ1126
IJ1127
IJ1126 lacUV5 lacZ::T7 gene1-KnrSee Endy:IJ1127
JM83
rpsL ara Δ(lac-proAB) Φ80dlacZΔM15
Sigma lists thi. Check this.streptomycin resistant
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JM101
glnV44 thi-1 Δ(lac-proAB) F'[lacIqZΔM15 traD36 proAB+]
host for M13mp vectorsrecA+, rK+
original blue/white cloning strainhas all wt restriction systems
References: Messing, J. et al. (1981) Nucleic Acids Res. 9, 309; Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Gene 33,103.
JM103
endA1 glnV44 sbcBC rpsL thi-1 Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZΔM15]
streptomycin resistant
References: Hanahan, D. (1983) J. Mol. Biol. 166:557-80.
NEB says this strain encodes a prophage encoded EcoP1 endonuclease.Sigma lists (P1) (rK-mK+ rP1+ mP1+)
JM105
endA1 glnV44 sbcB15 rpsL thi-1 Δ(lac-proAB) [F' traD36 proAB+ lacIq lacZΔM15] hsdR4(rK-mK+)
Sigma lists sbcC
streptomycin resistant
References: Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Gene 33, 103.
JM106
endA1 glnV44 thi-1 relA1 gyrA96 Δ(lac-proAB) F- hsdR17(rK-mK+)
References: Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Gene 33, 103.
JM107
endA1 glnV44 thi-1 relA1 gyrA96 Δ(lac-proAB) [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(RK- mK+) λ-host for M13mp vectorsrecA+, rK+
Sigma lists e14- (McrA-)nalidixic acid resistant
References: Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Gene 33, 103.
JM108
endA1 recA1 gyrA96 thi-1 relA1 glnV44 Δ(lac-proAB) hsdR17 (rK- mK+)
nalidixic acid resistant
deficient in expression of the lon protease due to IS186 transposon insertion -- J Mairhofer 18:59, 24 March 2010 (CET)3. Mairhofer J, Cserjan-Puschmann M, Striedner G, Nöbauer K, Razzazi-Fazeli E, and Grabherr R. . pmid:20138928.
JM109
endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)
From NEB (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/ecoli_genotypes.asp)Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–).
Suppresses many amber mutations when glutamine is acceptable but not the S100 or S7 mutations of λ, e.g., λgt11.Can also be used for M13 cloning/sequencing and blue/white screening.Sigma lists e14-nalidixic acid resistant
deficient in expression of the lon protease due to IS186 transposon insertion -- J Mairhofer 18:59, 24 March 2010 (CET)
From C. Yanisch-Perron, J. Vieira, and J. Messing. Improved M13 phage cloning vectors and host strains: nucleotide sequences ofthe M13mp18 and pUC19 vectors. Gene, 33(1):103–19, 1985.
Some information from Mary Berlyn at the E. coli Genetic Stock Center (http://cgsc.biology.yale.edu/) : One of the reasons theoriginal curator of this collection did not accession the JM109, JM103, etc. strains was because she found it impossible to besure of the derivation and therefore the details of the genotype. But I think it's safe to assume that the F' in this strain isderived from or similar to F128 which extends from the proBA region through the lac operon. It thus carries the wildtype genesfor all loci in that region except those indicated as mutant for the genotype of the F'. So it carries the lacZ (alpha-complementation) deletion lacZ58(M150 and the lacI mutation lacIq, but it has the lacY+ gene also on the F-prime. On thechromosome it lacks all the lac operon genes.
NOTE: The promoter driving the expression of lacI was sequenced in this strain using a primer in mhpR (upstream of lacI) and a primerin the opposite orientation in lacI. The lac promoter was found to be identical to wildtype. Thus, the -35 sequence was GCGCAA notGTGCAA as expected with lacIQ. Therefore this strain (or at least the version we have) does NOT appear to be lacIQ unless there is
another copy of lacI elsewhere. This result is somewhat confirmed by the fact that a lacI regulated promoter driving expression of YFPon a medium copy vector does not repress completely. -Reshma 13:48, 5 May 2005 (EDT)
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JM109(DE3)
JM109 + λ(DE3)
DE3 prophage carrying T7 polymerase expression cassette
Same cassette as BL21(DE3) carrying a lac inducible T7 RNA polymerase and lacIqnalidixic acid resistant
JM110
rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 Δ(lac-proAB) e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)
Sigma fails to list tonA tsx e14 fhuA hsdR17(e14-) status uncertainstreptomycin resistant
JM2.300
lacI22, LAM-, e14-, rpsL135(strR), malT1(LamR), xyl-7, mtl-1, thi-1
Some folks have been using this strain (i.e., Elowitz, Gardner) and it took me too long to find the CGSC#.This strain is no longer available from the CGSC
This strain may also be F-. JM2.300 is described as F-, lacI22, λ-, e14-, rpsL135(StrR), thi-1 in K. Brenner, D. Karig, R.Weiss, F. Arnold, “Engineered biodirectional communication mediates a consensus in a microbial biofilm consortium,” in Proc.Natl. Acad. Sci. USA, 2007 Oct 30;104(44):17300-4.
LE392
glnV44 supF58 (lacY1 or ΔlacZY) galK2 galT22 metB1 trpR55 hsdR514(rK-mK+)
Sigma lists F- e14-
M15 (Qiagen)
F-, Φ80ΔlacM15, thi, lac-, mtl-, recA+ , KmR
From Qiagen
E. coli M15 DZ291
Qiagen variant includes the pREP4 plasmid which confers kanamycin resistance and constitutively expresses the lac repressorprotein encoded by the lac I gene.
M15 cannot be infected by lambda phages
PL promoter introduced; however, is not activeReferences:
QIAexpress® Data Sheet: E. coli strain M15 - (EN) (http://www.qiagen.com/resources/resourcedetail?id=73e1f246-204e-47a8-8a9c-b8a808870c2a&lang=en)
Villarejo, M.R. and Zabin, I. (1974) J. Bacteriol. 120, 466. PMID 4607501.
Mach1
ΔrecA1398 endA1 tonA Φ80ΔlacM15 ΔlacX74 hsdR(rK- mK+)
From Invitrogen
Doubling time approx. 50 min and supposedly fastest growing chemically competent cloning strain available
Mach1 cells are derivatives of E. coli W strains (ATCC 9637, S. A. Waksman), rather than E. coli K-12. This may have implicationsfor BL-1 status for some facilities (apparently not for MIT).
See Bloom04 patent for details on the construction and properties of this strain.
MC1061
F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(r-m+)
Streptomycin resistant
The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain construction.Parent of DH10B/TOP10 and derived strainsReferences:
E. coli Genetic Stock Center, MC1061 Record (http://cgsc2.biology.yale.edu/Strain.php?ID=11225)Casdaban, M. and Cohen, S. (1980) J Mol Biol 138:179 PMID 6997493.Complete DH10B sequence is available, see Durfee08, PMID 18245285.
MC4100
F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1)deoC1
The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain construction.
This paper (http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12618467) compares MC4100 to MG1655 anddescribes the significant deletions.
*The paper referenced above showed that this deletion was larger than previously known. The deletion now covers ykfD-b0350.‡The fruA25 allele is attributed to the deletion of fruK-yeiR. This means fruA is present but its promoter has been deleted.
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The paper also shows that the e14 element is deleted in MC4100. One of the genes removed by this deletion is mcrA, which encodesan enzyme that restricts DNA containing methylcytosine. However, other E. coli K-12 restriction/modification systems are stillpresent in MC4100. MC4100 still encodes the McrBC 5-methylcytosine=specific restriction enzyme and the HsdR/HsdS/HsdM type Irestriction-modification complex.
Table three of the paper lists all genes believed to be deleted in MC4100. The methods used in the paper can detect deletions butnot loss of function mutations.
See CGSC#6152 (http://cgsc2.biology.yale.edu/Strain.php?ID=9973)
MFDpir
MG1655 RP4-2-Tc::[ΔMu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] ΔdapA::(erm-pir) ΔrecA
E. coli strain for performing biparental mating to transfer plasmids to other bacteria. This strain was constructed after noticingthat the common conjugation strains, SM10 and S17-1, were Hfr+ and also transferring a Mu transposon to the recipient strain.Refer >> Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed UsingSuicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machineryhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008518/
MG1655
F- λ- ilvG- rfb-50 rph-1
This is the \"wild type\" K-12 strain which was sequenced, and should be used when PCRing genes from the sequenced genome. It also looksvery healthy under the microscope -- a dramatic difference from most of the cloning strains, which appear sick.
See CGSC#6300 (http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/4837)See ATCC 700926
4. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J,
Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, and Shao Y. . pmid:9278503.
More accurate sequence correcting 243 errors in the original sequencing[5]. New Genbank accession number U00096.2
Latest genome sequence can be found with Genbank accession number U00096.3
OmniMAX2
From Invitrogen: \"This strain overexpresses the Lac repressor (lacIq gene). For blue/white screening, you will need to add IPTG toinduce expression from the lac promoter. Strain is resistant to T1 bacteriophage.\"
F′ {proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80(lacZ)ΔM15 Δ(lacZYA-argF) U169 endA1 recA1 supE44thi-1 gyrA96 relA1 tonA panD
OverExpress(tm)C41(DE3) (Lucigen)
F– ompT gal dcm hsdSB(rB- mB-)(DE3)
The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strainC41(DE3) was derived from BL21(DE3). This strain has at least one uncharacterized mutation, which prevents cell death associatedwith expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance toa different toxic protein and can express a different set of toxic proteins to C41(DE3).an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
OverExpress(tm)C41(DE3)pLysS (Lucigen)
F– ompT gal dcm hsdSB(rB- mB-)(DE3)pLysS (Cmr)
The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strainC41(DE3) was derived from BL21(DE3). This strain has at least one uncharacterized mutation, which prevents cell death associatedwith expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance toa different toxic protein and can express a different set of toxic proteins to C41(DE3).an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression fromtransformed T7 promoter containing plasmids when not induced.
OverExpress(tm)C43(DE3) (Lucigen)
F– ompT gal dcm hsdSB(rB- mB-)(DE3)
The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strainC41(DE3) was derived from BL21(DE3). This strain has at least one uncharacterized mutation, which prevents cell death associatedwith expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance toa different toxic protein and can express a different set of toxic proteins to C41(DE3).an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
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OverExpress(tm)C43(DE3)pLysS (Lucigen)
F– ompT gal dcm hsdSB(rB- mB-)(DE3)pLysS (Cmr)
The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strainC41(DE3) was derived from BL21(DE3). This strain has at least one uncharacterized mutation, which prevents cell death associatedwith expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance toa different toxic protein and can express a different set of toxic proteins to C41(DE3).an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression fromtransformed T7 promoter containing plasmids when not induced.
Rosetta(DE3)pLysS
F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)
an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
Chloramphenicol resistant
pLysSRARE contains tRNA genes argU, argW, ileX, glyT, leuW, proL, metT, thrT, tyrU, and thrU. The rare codons AGG, AGA, AUA, CUA,CCC, and GGA are supplemented.
The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression fromtransformed T7 promoter containing plasmids when not induced.see Moffatt87 for details of pLysS and pLysE plasmids
Novagen strain manual (http://www.emdbiosciences.com/Search/gsr.asp?criteria=strain+manual&x=22&y=11)
Rosetta-gami(DE3)pLysS
Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F'[lac+ lacIq pro] gor522::Tn10 trxB pLysSRARE (CamR,StrR, TetR)
an E. coli K-12 strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lacpromoter.
ahpC mutation allows trxB/gor double mutants to grow in the absence of reducing medium
pLysSRARE contains tRNA genes argU, argW, ileX, glyT, leuW, proL, metT, thrT, tyrU, and thrU. The rare codons AGG, AGA, AUA, CUA,CCC, and GGA are supplemented.
The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression fromtransformed T7 promoter containing plasmids when not induced.see Moffatt87 for details of pLysS and pLysE plasmidsChloramphenicol resistantKanamycin resistantTetracycline resistantStreptomycin resistant
Novagen strain manual (http://www.clinalfa.com/docs/docs/PROT/TB009.pdf)
RR1
HB101 recA+
RV308
lacIq-, su-, ΔlacX74, gal IS II::OP308, strA K12 derivative used for industrial protein production. ATCC strain 31608, deposited byGenentech. Complete Genome Sequence available LM995446 (http://www.ebi.ac.uk/ena/data/view/LM995446/) *J Mairhofer 07:36, 28 October2014 (EDT):.
SOLR (Stratagene)
e14-(McrA-) Δ(mcrCB-hsdSMR-mrr)171 sbcC recB recJ uvrC umuC::Tn5 (Kanr) lac gyrA96 relA1 thi-1 endA1 λR [F’ proAB lacIqZ ΔM15]C Su-Used in phagemid recovery (LambdaZap)Kanamycin resistant
Stratagene E. coli Genotype Strains (http://www.stratagene.com/pdf/mobio/E%20Coli_Genotype%20Strains.pdf)
SS320 (Lucigen)
F'[proAB+lacIqlacZΔM15 Tn10 (tetr)]hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galUgalK rpsL thi
Useful for phage display.
Sidhu, S.S., Weiss, G.A., and Wells, J.A. (2000) J. Mol. Biol. 296, 487-495.
STBL2 (Invitrogen)
F- endA1 glnV44 thi-1 recA1 gyrA96 relA1 Δ(lac-proAB) mcrA Δ(mcrBC-hsdRMS-mrr) λ-host for unstable sequences such as retroviral sequences and direct repeats
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nalidixic acid resistant
References: Trinh, T., Jessee, J., Bloom, F.R., and Hirsch, V. (1994) FOCUS 16, 78.
STBL3 (Invitrogen)
F- glnV44 recA13 mcrB mrr hsdS20(rB-, mB-) ara-14 galK2 lacY1 proA2 rpsL20 xyl-5 leu mtl-1
Streptomycin resistant
endA+, use care in preparing DNA from this strain
STBL4
endA1 glnV44 thi-1 recA1 gyrA96 relA1 Δ(lac-proAB) mcrA Δ(mcrBC-hsdRMS-mrr) λ- gal F'[ proAB+ lacIq lacZΔM15 Tn10]
Tetracycline resistant (from Tn10 insertion)STBL2 + blue/white selection
SURE (Stratagene)
endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- Δ(mcrCB-hsdSMR-mrr)171 F'[ proAB+ lacIq lacZΔM15 Tn10]
uncertain status of TraD36 in F plasmid
increased stability for inverted repeats and Z-DNAnalidixic acid resistantkanamycin resistanttetracycline resistant
SURE2 (Stratagene)
endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- Δ(mcrCB-hsdSMR-mrr)171 F'[ proAB+ lacIq lacZΔM15 Tn10 AmyCmR]
increased stability for inverted repeats and Z-DNAnalidixic acid resistantkanamycin resistanttetracycline resistant
chloramphenicol resistant for < 40 μg/ml, sensitive for > 100 μg/ml
TG1 (Lucigen)
F' [traD36 proAB+ lacIq lacZΔM15]supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5, (rK-mK-)
Useful for phage display.
TOP10 (Invitrogen)
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-Very similar to DH10B
I actually emailed Invitrogen and asked if DH10B and TOP10 are the same strain or what. Their response: \"Thank you forcontacting Invitrogen Technical Support.TOP10 and DH10B competent cells are closely related. They have the same genotypesand can used for the same applications. You can also choose from those that are Chemically competent or electrocomp cells. Ihope this information answers your questions.\" So either there is a difference that they don't want to put out there, orthey have rebranded DH10B as TOP10 for marketing purposes... --Dcekiert 18:55, 23 January 2008 (CST)
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B (and bytheir lineage also TOP10 and any other MC1061 derivatives) is actually galE galK galU+. --Dcekiert 16:45, 23 January 2008 (CST)Previously reported to be deoR, but genome sequence indicates that DH10B is actually deoR+. Presumably TOP10 and MC1061 are alsodeoR+.
Streptomycin resistantan MC1061 derivative [6]
Prepare cells for chemical transformation with CCMB80 buffer Here
Contain lacI based on a colony PCR (even though lacX74 supposedly deletes the lac operon) --Austin Che 16:16, 18 June 2007 (EDT)
φ80lacZΔM15 actually contains the entire lac operon, including lacIq --Dcekiert 16:45, 23 January 2008 (CST)
Analysis of the published DH10B sequence (Genbank CP000948) suggests the φ80lacZΔM15 insertion has the wild-type lacI-35 sequence, not the lacIq -35 sequence (gtgcaa) --BC 15:01, 29 March 2008 (EDT)
leucine auxoroph (source (http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/competent-cells-for-transformation/competent-cells-resources/genotypes-of-competent-cells.html) )References:
E. coli Genetic Stock Center, MC1061 Record (http://cgsc2.biology.yale.edu/Strain.php?ID=11225)
DH10B Genome Sequencing Project, Baylor College of Medicine (http://www.hgsc.bcm.tmc.edu/projects/microbial/microbial-detail.xsp?project_id=105)6. Casadaban MJ and Cohen SN. . pmid:6997493.
7. Durfee T, Nelson R, Baldwin S, Plunkett G 3rd, Burland V, Mau B, Petrosino JF, Qin X, Muzny DM, Ayele M, Gibbs RA, Csörgo B,
Pósfai G, Weinstock GM, and Blattner FR. . pmid:18245285.
Complete DH10B sequence is available
8. Grant SG, Jessee J, Bloom FR, and Hanahan D. . pmid:2162051.
Top10F' (Invitrogen)
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F'[lacIq Tn10(tetR)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR)endA1 λ-Very similar to DH10B with F plasmid containing lacIq and Tn10
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B (and bytheir lineage also TOP10 and any other MC1061 derivatives) is actually galE galK galU+. --Dcekiert 16:45, 23 January 2008 (CST)Previously reported to be deoR, but genome sequence indicates that DH10B is actually deoR+. Presumably TOP10 and MC1061 are alsodeoR+.
Tetracycline resistantStreptomycin resistantan MC1061 derivative [6]References:
E. coli Genetic Stock Center, MC1061 Record (http://cgsc2.biology.yale.edu/Strain.php?ID=11225)
DH10B Genome Sequencing Project, Baylor College of Medicine (http://www.hgsc.bcm.tmc.edu/projects/microbial/microbial-detail.xsp?project_id=105)
Complete DH10B sequence is available, see Durfee08, PMID 18245285.
W3110
F- λ- rph-1 INV(rrnD, rrnE)
See CGSC#4474 (http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/5446)See ATCC 39936
See [9]. Briefly, there are 8 site (9nt) differences between W3110 and MG1655. They reside in 7 orgs and one rRNA gene. Two arenonfunctional (rpoS and dcuA) and 5 are unknown missense mutations.New annotation has accession number DDBJ AP009048.
W3110 (λ857S7)
Phenotype: ?
See also the Talk page and the \"W3110 (λ857S7)\" section to discuss about this E. coli/K-12 strain.
WM3064
thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir]
Derived from B2155. Is auxotrophic to DAP (see strain information for B2155). This strain can be used for conjugation experiments andreplication of plasmids that require pir protein.Strain developed by William Metcalf at UIUC.
XL1-Blue (Stratagene)
endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)
nalidixic acid resistant
tetracycline resistant (carried on the F plasmid)
XL1-Blue MRF' (Stratagene)
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]
Used in lambda phage infection, amplification, expressionTetracyline resistant
Stratagene E. coli Genotype Strains (http://www.stratagene.com/pdf/mobio/E%20Coli_Genotype%20Strains.pdf)
XL2-Blue (Stratagene)
endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15 Amy CmR] hsdR17(rK- mK+)
nalidixic acid resistant
tetracycline resistant (carried on the F plasmid)
chloramphenicol resistant for <40 μg/ml; sensitive for >100 μg/ml
XL2-Blue MRF' (Stratagene)
endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 e14- Δ(mcrCB-hsdSMR-mrr)171 recB recJ sbcC umuC::Tn5 uvrC F'[ ::Tn10 proAB+ lacIqΔ(lacZ)M15 Amy CmR]
Minus Restriction strain (minus mcrA mcrCB mcrF mrr hsdR)nalidixic acid resistantkanamycin resistant
tetracycline resistant (carried on the F plasmid)
chloramphenicol resistant <40 μg/ml, sensitive >100μg/ml
XL1-Red (Stratagene)
F- endA1 gyrA96(nalR) thi-1 relA1 lac glnV44 hsdR17(rK- mK+) mutS mutT mutD5 Tn10
nalidixic acid resistant
http://openwetware.org/wiki/E._coli_genotypes
13/14
2015/5/26E. coli genotypes OpenWetWare
tetracycline resistant
mutator strain, produces highly unstable DNA changes
colonies grow and mutate so quickly that the strain is sick and mutated constructs must be moved rapidly to stable strains forplasmid isolation
XL10-Gold (Stratagene)
endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 tetR F'[proAB lacIqZΔM15 Tn10(TetR Amy CmR)]
Tetracycline and Chloramphenicol resistantNalidixic acid resistant
Hte phenotype allows high transformation with large plasmid inserts
XL10-Gold KanR (Stratagene)
endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 tetR F'[proAB lacIqZΔM15 Tn10(TetR Amy Tn5(KanR)]
Tetracycline and Kanamycin resistantNalidixic acid resistant
Hte phenotype allows high transformation with large plasmid inserts
Other genotype information sources
Bachmann B, Bacteriol Rev. 1972 Dec;36(4):525-57. Pedigrees of some mutant strains of Escherichia coli K-12. PMID 4568763
History of the derivation of most lab strains of E. coliStrains at EcoliWiki.org
Provides information about common E. coli laboratory strains, allowing for annotation of the genotype, plasmids, phages andsource information of a particular strain.E. coli Genetic Stock Center (http://cgsc2.biology.yale.edu/index.php)
E. coli genotypes/Exhibit: Test of moving information on this page into a wiki database
NEB strains (http://www.neb.com/nebecomm/products/category64.asp?) Other NEB strains(http://www.neb.com/nebecomm/products/category95.asp)
NEB genotype information (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/genetic_markers.asp)Teknova (http://216.247.204.235/technical-main.html)
Promega (http://www.promega.com/guides/cloning_guide/appendix.pdf)
EMBL (http://www.embl-hamburg.de/~geerlof/webPP/straindb/bact_strains/bact_strains.html)Novagen/EMD (http://www.emdbiosciences.com/docs/docs/PROT/TB009.pdf)Sigma
(http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Molecular_Biology/Cloning_and_Expression/Key_Resources/Genotypes_of_E_coli.htmEcoCyc (http://EcoCyc.org) , EcoCyc Query Page (http://biocyc.org/server.html) , and EcoCyc Genome Browser(http://biocyc.org/ECOLI/NEW-IMAGE?chromosome=COLI-K12&type=LOCUS-POSITION&bp-range=1%2F50000&x=21&y=41)EcoliWiki strains (http://ecoliwiki.net/colipedia/index.php/Category:Strains) and EcoliWiki home(http://ecoliwiki.net/colipedia/index.php/Welcome_to_EcoliWiki)
Escherichia coli K12 genome browser (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genome&cmd=Retrieve&dopt=Overview&list_uids=115)
References
9. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, and Horiuchi T. .
pmid:16738553.
10. Novick RP, Clowes RC, Cohen SN, Curtiss R 3rd, Datta N, and Falkow S. . pmid:1267736.
11. Lim A, Dimalanta ET, Potamousis KD, Apodaca J, Ananthara-man TS, and Witkin, EM. Inherited differences in sensitivity to
radiation in Escherichia coli. Proc Natl Acad Sci USA 1946 32:59-68 (the original B strain reference).12. Moffatt BA and Studier FW. . pmid:3568126.
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