北京华越洋VECT75604
pAAV-IRES-ZsGreen1载体基本信息：启动子:复制子:终止子:质粒分类:质粒大小:原核抗性:克隆菌株:培养条件:表达宿主:诱导方式:5'测序引物:3'测序引物:CMVpUCori，f1orihGHpoly(A)signal病毒系列，腺病毒相关克隆载体5904bpAmpStbl337℃，有氧LB哺乳细胞无须诱导，瞬时表达CMV-F：CGCAAATGGGCGGTAGGCGTGhGH-PA-R:CCAGCTTGGTTCCCAATAGA载体名称
pAAV-IRES-ZsGreen1
pAAV-IRES-ZsGreen1载体图谱：
pAAV-IRES-ZsGreen1载体简介：
ThepAAV-ZsGreen1Vectorisanadeno-associatedvirus(AAV)vectorthatcontainstheZsGreen1gene.ThisvectorcanbeusedasapositivecontroltomonitorAAVparticleproductionusingAAVproHelperFreeSystems.ExpressionofZsGreen,avariantofthegreenfluorescentproteinfromZoanthussp.,canbeusedtoassesstransfectionandtransductionefficiencyduringAAVparticlepreparation.pAAV-IRES-ZsGreen1载体序列：
ORIGIN1cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtc61gggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggcca121actccatcactaggggttcctgcggccgcacgcgtggagctagttattaatagtaatcaa181ttacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaa241atggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatg301ttcccatagtaacgtcaatagggactttccattgacgtcaatgggtggagtatttacggt361aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacg421tcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttc481ctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggc541agtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccacccca601ttgacgtcaatgggagtttgttttgcaccaaaatcaacgggactttccaaaatgtcgtaa661caactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataag721cagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacct781ccatagaagacaccgggaccgatccagcctccgcggattcgaatcccggccgggaacggt841gcattggaacgcggattccccgtgccaagagtgacgtaagtaccgcctatagagtctata901ggcccacaaaaaatgctttcttcttttaatatacttttttgtttatcttatttctaatac961tttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctctttgcacc1021attctaaagaataacagtgataatttctgggttaaggcaatagcaatatttctgcatata1081aatatttctgcatataaattgtaactgatgtaagaggtttcatattgctaatagcagcta1141caatccagctaccattctgcttttattttatggttgggataaggctggattattctgagt1201ccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctg1261ggcaacgtgctggtctgtgtgctggcccatcactttggcaaagaattgggattcgaacat1321cgattgaattccccggggatcccgcccctctccctcccccccccctaacgttactggccg1381aagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgcc1441gtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctag1501gggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagt1561tcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaa1621ccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgc1681aaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatg1741gctcacctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtat1801gggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaa1861cgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatat1921ggcccagtccaagcacggcctgaccaaggagatgaccatgaagtaccgcatggagggctg1981cgtggacggccacaagttcgtgatcaccggcgagggcatcggctaccccttcaagggcaa2041gcaggccatcaacctgtgcgtggtggagggcggccccttgcccttcgccgaggacatctt2101gtccgccgccttcatgtacggcaaccgcgtgttcaccgagtacccccaggacatcgtcga2161ctacttcaagaactcctgccccgccggctacacctgggaccgctccttcctgttcgagga2221cggcgccgtgtgcatctgcaacgccgacatcaccgtgagcgtggaggagaactgcatgta2281ccacgagtccaagttctacggcgtgaacttccccgccgacggccccgtgatgaagaagat2341gaccgacaactgggagccctcctgcgagaagatcatccccgtgcccaagcagggcatctt2401gaagggcgacgtgagcatgtacctgctgctgaaggacggtggccgcttgcgctgccagtt2461cgacaccgtgtacaaggccaagtccgtgccccgcaagatgcccgactggcacttcatcca2521gcacaagctgacccgcgaggaccgcagcgacgccaagaaccagaagtggcacctgaccga2581gcacgccatcgcctccggctccgccttgccctgaaagcttgcctcgagcagcgctgctcg2641agagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagt2701tgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctga2761ctaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagt2821tgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggc2881acaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcc2941tcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttg3001gtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgat3061ctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccc3121tgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtg3181atggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaag3241gtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgc3301ctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgc3361atacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtgg3421tggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctt3481tcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggc3541tccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgg3601gtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttgg3661agtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatct3721cgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatg3781agctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttat3841ggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgc3901caacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaag3961ctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcg4021cgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatgg4081tttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttat4141ttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttc4201aataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccct4261tttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaag4321atgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggta4381agatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttc4441tgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgca4501tacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacgg4561atggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcgg4621ccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca4681tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaa4741acgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaa4801ctggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggata4861aagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaat4921ctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagc4981cctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaata5041gacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagttt5101actcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtga5161agatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgag5221cgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaa5281tctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaag5341agctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactg5401tccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacat5461acctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtctta5521ccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggg5581gttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagc5641gtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaa5701gcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatc5761tttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgt5821caggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggcct5881tttgctggccttttgctcacatgt//pAAV-IRES-ZsGreen1载体其他相关的腺病毒载体：pAdTrack-CMVpAdTrackpShuttle-CMVpAdEasy-1pHelper
pAAV-IRES-ZsGreen1pAAV-IRES-hrGFPpAAV-RCpAAV-MCS
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