专利名称：Detection and characterization of
microorganisms
发明人：Norman G. Anderson,N. Leigh Anderson申请号：US09265541申请日：19990309公开号：US06254834B1公开日：20010703
专利附图：
摘要：A method for separating microorganisms, especially infectious agents, from amixture by two dimensional centrifugation on the basis of sedimentation rate andisopycnic banding density, for sedimenting such microorganisms through zones of
immobilized reagents to which they are resistant, for detecting banded particles by lightscatter or fluorescence using nucleic acid specific dyes, and for recovering the bandedparticles in very small volumes for characterization by mass spectrometry of viral proteinsubunits and intact viral particles, and by fluorescence flow cytometric determination ofboth nucleic acid mass and the masses of fragments produced by restriction enzymes.The method is based on the discovery that individual microorganisms, such as bacterialand viral species, are each physically relatively homogeneous, and are distinguishable intheir biophysical properties from other biological particles, and from non-biologicalparticles found in nature. The method is useful for distinguishing infections, foridentifying known microorganisms, and for discovering and characterizing new
microorganisms. The method provides very rapid identification of microorganisms, andhence allows a rational choice of therapy for identified infectious agents. A particularlyuseful application is in clinical trials of new antibiotics and antivirals, where it is essentialto identify at the outset individuals infected with the targeted infectious agent.
申请人：LARGE SCALE PROTEOMICS CORP.
代理机构：Rothwell, Figg, Ernst & Manbeck
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