专利名称：Compositions and methods for identifying
bacterial clonotypes and detecting antibioticsusceptibility
发明人：Sujay Chattopdhyay申请号：US15055376申请日：20160226公开号：US09970063B2公开日：20180515
摘要：There is disclosed a PCR-based test kit and PCR process for identification ofmultiple clonal sub-species lineages of infectious bacteria, such as uropathogenic causingcystitis. pyelonephritis and urosepsis, for the purposes of predicting antibiotic resistanceof the bacteria. More specifically, there is further disclosed a SNP (single nucleotidepolymorphism) identification process that simultaneously detect compilations of thepresence of absence of predictive SNPs within mutated loci of infectious bacterial clonalsubspecies variants, such as the fumC/fimH loci of the bacterium. This disclosure providesa PCR detection kit incorporating a SNP compilation that forms a BFC (Binary FootprintCode) that allows for rapid identification of multiple infectious bacterial clonotypesbased on their SNP footprint. More specifically there is disclosed a clonotyping methodfor clonal typing and predicting antibiotic susceptibility, comprising (a) providing forwardprimers and reverse primers for at least seven SNPs (single nucleotide polymorphisms)selected from the group consisting of fumC-63, fumC-248, fumC-380, fimH-162, fimH-233,fimH-483, and fimH-108, (b) measuring the presence or absence of each SNP, and (c)determining antibiotic susceptibility from Lookup Table 1.
申请人：ID Genomics, Inc.
地址：Seattle WA US
国籍：US
代理机构：Seed IP Law Group LLP
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