Methods and compositions for biotechnical separati

专利名称：Methods and compositions for biotechnical

separations using selective precipitation bycompaction agents

发明人：Richard C. Willson, III,Jason Murphy申请号：US09609996申请日：20000703公开号：US06617108B1公开日：20030909

摘要：Preferred embodiments of the invention include purification of DNA, preferablyplasmid DNA, by use of selective precipitation, preferably by addition of compactionagents. Also, included is a sealable method for the liquid phase separation of DNA fromRNA. RNA may also be recovered by fractional precipitation according to the invention.Applicants have discovered that RNA, commonly the major contaminant in DNApreparations, can be left in solution while valuable purified plasmid DNA is directlyprecipitated. Additional aspects of the invention include mini-preps, preferably of plasmidand chromosomal DNA, to obtain sequenceable and restriction digestible DNA in highyields in multiple simultaneous procedures. Still further aspects disclose enhancedstripping of the compaction agent by a stripping method comprising high salt additionand pH shift, and combinations of these techniques. Also, disclosed is a method of assayin which a labeled probe is precipitated when it is hybridized to a target, (e.g.

chromosomal DNA, oligonuclotides, Ribosomal RNA, tRNA), and thereafter precipitatingthe probe/target complex with compaction agents and leaving in solution anyunhybridized probe. For example, chromosomal DNA, plasmid, ribosomal RNA, and

oligonucleotides can be recovered in excellent purity; by then heating the mixture ofnucleic acids (above their melting temperature if the hybridization site is buried withinsecondary structure) and thereafter precipitating the probe and the target.

申请人：TECHNOLOGY LICENSING CO. LLC

代理人：Richard Coale Willson, Jr.

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