DNA probe signal amplification

专利名称：DNA probe signal amplification发明人：Segev, David申请号：EP91105249.6申请日：19910403公开号：EP0450594A2公开日：19911009

专利附图：

摘要：The invention relates to a method for amplifying a signal during the detectionof target nucleic acid molecules comprising the steps of: a) hybridizing a first sequence ofa primary oligonucleotide probe to the target nucleic acid molecules wherein the primaryprobe has a means for binding to a bridging nucleic acid molecule, the bridging moleculebeing capable of hybridizing to at least one developer nucleic acid molecule;b) exposingthe primary probe to the bridging molecule and to a first developer molecule and asecond developer molecule, each developer molecule comprising: i) a first branchcomprising a sequence of at least two different nucleotides and at least six totalnucleotides complementary to a sequence of a first branch of the other developermolecule;ii) a second branch comprising a sequence of at least two different nucleotidesand at least six total nucleotides complementary to a sequence of a second branch of the

other developer molecule; andiii) a detectable label or a means of being converted into adeveloper molecule comprising a detectable label;    wherein the first developermolecule and the second developer molecule have structures that are the same ordifferent; and    steps a and b being conducted under conditions such that: i) thebridging molecule binds to the primary probe and hybridizes to the first developermolecule; andii) the bound first developer molecule hybridizes to the second developermolecule to form a developer chain; andc) detecting the labeled developer molecule inthe developer chain

申请人：Segev, David

地址：1125 52nd Street Brooklyn, N.Y. 11219 US

国籍：US

代理机构：Grünecker, Kinkeldey, Stockmair & Schwanhäusser Anwaltssozietät

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